Government funding of £30 million is to be used to help 13 local authorities and bus operators buy low emission vehicles.
More than 320 electric, hybrid, hydrogen and biomethane buses will be added to fleets across the UK.
The money will also be used by the 13 successful bidders to install chargeing points and other infrastructure costing more than £7 million.
Bus passengers will benefit from cleaner, greener journeys thanks to the funding, confirmed today by Transport Minister Andrew Jones.
Low emission buses produce at least 15% fewer greenhouse gas emissions than a diesel bus but they cost significantly more. The funding will cover up to 90% of the difference in cost between a new bus and its diesel equivalent as well as up to 75% of the cost of infrastructure.
Among the winners is Sheffield City Region, which has been awarded £1.3 million for 44 buses fitted with hybrid technology.
Transport Minister Andrew Jones said: “Low emission buses have already proved to be a real success across the country. They are cost efficient, good for the environment and there are wider benefits.
“We have provided more than £2 billion of funding to greener transport schemes since 2011 and by supporting this technology the government is ensuring the UK is driving innovation and investment up and down the country.”